Interfaculty cooperation with the scientific team of associate professor Zdenko Machala from the Faculty of Mathematics, Physics and Informatics; by the bioinformatics team of Dr Tomáš Szemeš from the Comenius University Science Park and our colleagues under the leadership of associate professor Roman Gardlík, it was transformed into a publication. The obtained results did not support the therapeutic potential of plasma-activated water (PAW) in ulcerative colitis from the point of view of oxidative stress or microbial diversity. However, the application of PAW led to increased microbial diversity in healthy individuals, which opens up other interesting perspectives for the use of PAW in biomedicine.