On 27 October 2026, the first edition of the Bratislava Oxford Nanopore Day 2026 (BONDay 2026) professional symposium took place at the Science Park of Comenius University in Bratislava. The aim of the event was to create a space for current and future users of Oxford Nanopore Technologies to meet, present their ongoing projects, exchange practical experience, and foster new scientific and professional collaborations. From our team, Doctor Silvia Bendíková presented her analysis of the gut microbiome using 16S rRNA nanopore sequencing, and PhD student Tomáš Strečanský presented the uroPARADiX acute project – ultra-rapid identification of the causative agents of urinary tract infections (UTIs) at the emergency department of BORY Hospital.