Extracellular DNA analysis:

  1. RT-PCR, PCR
  2. Fluorometrically

DNase activity analysis:

  1. Single radial enzyme diffusion (SRED)

Neutrophil assays:

  1. Microbiological techniques
  2. Antimicrobial assay (bactericidal assay)

Flow cytometry assays:

  1. Immunophenotyping of white blood cells
  2. Neutrophil production of extracellular traps, reactive oxygen species
  3. Intracellular calcium flux
  4. Detection and determination of microparticles and mitochondria in body fluids
  5. Mitochondrial membrane potential

Live cell imaging and fluorescent microscopy assays:

  1. Visualization of NETs formation in time
  2. Detection of NETs-captured bacteria
  3. Cell chemotaxis

Behavioral tests evaluating:

  1. Motor strength, locomotor activity and explorative behavior
    • Negative geotaxis
    • Open field test
    • Test of novel object exposure
    • Social novelty test
  2. Anxiety-like behavior
    • Elevated zero-maze
    • Elevated plus maze
    • Light-dark box
  3. Depression-like behavior
    • Forced swim test
    • Sucrose preference test
    • Dplash test
  4. Cognitive abilities, learning and memory
    • Novel object recognition
    • T-maze
    • Morris water maze
  5. Sociability
    • Three-chamber sociability and social novelty test
    • Test of social interaction

Oxidative stress – biochemical measurement:

  1. TBARS assay (thiobarbituric acid reactive substances) – measurement of the lipid peroxidation product malondialdehyde
  2. AOPP assay (advanced oxidation protein products)
  3. Fructosamine assay – measurement of the sugar-amine glycation product fructosamine
  4. AGEs assay (advanced glycation end products) – measurement of glycated proteins or lipids
  5. GSH/GSSG assay (ratio of reduced and oxidised glutathione)
  6. TAC assay (total antioxidant capacity)
  7. FRAP assay (ferric reducing antioxidant power)