The project “Endogenous deoxyribonuclease activity and biology of extracellular DNA” (401101C202) focuses on research into extracellular DNA (ecDNA) and DNase enzymes that influence its degradation in the body. The aim is to better understand how differences in DNase activity between individuals affect the diagnostic use of ecDNA, for example in prenatal testing or oncology. The project combines experimental disease models, genetic approaches, and modern analytical methods to elucidate the biological and immunological effects of ecDNA and explore the potential of DNases as a therapeutic tool. The research is being conducted between 2025 and 2029 at the Clinical Research Unit of the 5th Internal Medicine Clinic of the Faculty of Medicine, Comenius University, at the National Institute of Endocrinology and Diabetology in Ľubochňa.